肽YY(3-36)和肽YY均由胃肠道合成并在餐后释放到循环中。肽 YY (3-36) 是一种 Y 2 受体亚型激动剂，而肽 YY 对 Y 1 和 Y 2 受体亚型没有选择性。已经提出 Y 1 和 Y 2 受体亚型结合亲和力取决于它们的二级和三级溶液状态结构。
编号：200293
CAS号：126339-09-1
单字母：H2N-AKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-CONH2
肽YY(3-36)和肽YY均由胃肠道合成并在餐后释放到循环中。肽 YY (3-36) 是一种 Y 2 受体亚型激动剂，而肽 YY 对 Y 1 和 Y 2 受体亚型没有选择性。已经提出 Y 1 和 Y 2 受体亚型结合亲和力取决于它们的二级和三级溶液状态结构。
Peptide YY (3-36) and peptide YY are both synthesized by the gastrointestinal tract and released into the circulation after a meal. Peptide YY (3-36) is a Y₂ receptor subtype agonist, whereas peptide YY is non-selective for Y₁ and Y₂ receptor subtypes. It has been suggested that Y₁ and Y₂ receptor subtype binding affinities depend on their secondary and tertiary solution state structures.
肽YY（PYY）（3-36），人类是一种肠道激素肽，充当Y2受体激动剂以降低食欲。
Peptide YY (PYY) (3-36), human is a gut hormone peptide that acts as a Y2 receptor agonist to reduce appetite.
人肽YY（PYY）（3-36）是一种Y2受体激动剂，通过二肽基肽酶IV（DPP-IV）切割PYY1-36 N末端的前两个氨基酸产生，可以减少食物摄入。
Peptide YY (PYY) (3-36), human is a Y2 receptor agonist, generated via cleaving the first two amino acids at the N terminus of PYY1-36 by enzyme dipeptidyl peptidase-IV (DPP-IV), and can reduces food intake.
在小鼠中，放线菌素显著延长了PYY3-36（50 nmol/kg）的厌食作用，并保持了比单独使用PYY3-36更高的PYY3-36-血浆水平。
In mice, actinonin significantly prolongs the anorectic effect of PYY3-36 (50 nmol/kg) and maintains higher PYY3-36 plasma levels than treatment with PYY3-36 alone.
| DOI | 名称 | |
|---|---|---|
| 10.1056/NEJMoa030204 | Inhibition of food intake in obese subjects by peptide YY3-36 | 下载 |
| 10.1152/ajpregu.00374.2003 | Stimulation of NPY Y2 receptors by PYY3-36 reveals divergent cardiovascular effects of endogenous NPY in rats on different dietary regimens | 下载 |
| 10.1016/j.cmet.2005.02.003 | Peripheral administration of PYY(3-36) produces conditioned taste aversion in mice | 下载 |
| 10.1038/sj.bjp.0706368 | The regulation of veratridine-stimulated electrogenic ion transport in mouse colon by neuropeptide Y (NPY), Y1 and Y2 receptors | 下载 |
| 10.1038/sj.ijo.0803137 | The importance of acclimatisation and habituation to experimental conditions when investigating the anorectic effects of gastrointestinal hormones in the rat | 下载 |
| 10.1677/joe.1.06784 | Peptide YY (PYY)3-36 modulates thyrotropin secretion in rats | 下载 |
| 10.1210/en.2006-1665 | Involvement of apolipoprotein A-IV and cholecystokinin1 receptors in exogenous peptide YY3 36-induced stimulation of intestinal feedback | 下载 |
| 10.1523/JNEUROSCI.5405-06.2007 | Peptide YY3-36 decreases reinstatement of high-fat food seeking during dieting in a rat relapse model | 下载 |
| 10.1152/ajpregu.00040.2008 | Effects of different intermittent peptide YY (3-36) dosing strategies on food intake, body weight, and adiposity in diet-induced obese rats | 下载 |
| 10.1111/j.1365-2826.2011.02111.x | Peptide YY 3-36 and pancreatic polypeptide differentially regulate hypothalamic neuronal activity in mice in vivo as measured by manganese-enhanced magnetic resonance imaging | 下载 |
| 10.1111/j.1476-5381.2011.01401.x | Endogenous peptide YY and neuropeptide Y inhibit colonic ion transport, contractility and transit differentially via Y₁ and Y₂ receptors | 下载 |
| 10.1038/ijo.2011.126 | Exogenous peptide YY3-36 and Exendin-4 further decrease food intake, whereas octreotide increases food intake in rats after Roux-en-Y gastric bypass | 下载 |
| 10.1371/journal.pone.0026137 | Salivary PYY: a putative bypass to satiety | 下载 |
| 10.1016/j.molmet.2013.03.001 | Peripheral activation of the Y2-receptor promotes secretion of GLP-1 and improves glucose tolerance | 下载 |
| 10.1523/JNEUROSCI.1064-13.2013 | Salivary peptide tyrosine-tyrosine 3-36 modulates ingestive behavior without inducing taste aversion | 下载 |
| 10.14814/phy2.12455 | The anorexic hormone Peptide YY3-36 is rapidly metabolized to inactive Peptide YY3-34 in vivo | 下载 |
| 10.1007/s10620-017-4788-3 | Peptide Tyrosine Tyrosine 3-36 Reduces Meal Size and Activates the Enteric Neurons in Male Sprague-Dawley Rats | 下载 |
多肽H2N-Ala-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2的合成步骤：
1、合成MBHA树脂：取若干克的MBHA树脂（如初始取代度为0.5mmol/g）和1倍树脂摩尔量的Fmoc-Linker-OH加入到反应器中，加入DMF，搅拌使氨基酸完全溶解。再加入树脂2倍量的DIEPA，搅拌混合均匀。再加入树脂0.95倍量的HBTU，搅拌混合均匀。反应3-4小时后，用DMF洗涤3次。用2倍树脂体积的10%乙酸酐/DMF 进行封端30分钟。然后再用DMF洗涤3次，甲醇洗涤2次，DCM洗涤2次，再用甲醇洗涤2次。真空干燥12小时以上，得到干燥的树脂{Fmoc-Linker-MHBA Resin}，测定取代度。这里测得取代度为 0.3mmol/g。结构如下图：
2、脱Fmoc：取2.78g的上述树脂，用DCM或DMF溶胀20分钟。用DMF洗涤2遍。加3倍树脂体积的20%Pip/DMF溶液，鼓氮气30分钟，然后2倍树脂体积的DMF 洗涤5次。得到 H2N-Linker-MBHA Resin 。（此步骤脱除Fmoc基团，茚三酮检测为蓝色，Pip为哌啶）。结构图如下：
3、缩合：取2.5mmol Fmoc-Tyr(tBu)-OH 氨基酸，加入到上述树脂里，加适当DMF溶解氨基酸，再依次加入5.0mmol DIPEA，2.38mmol HBTU。反应30分钟后，取小样洗涤，茚三酮检测为无色。用2倍树脂体积的DMF 洗涤3次树脂。（洗涤树脂，去掉残留溶剂，为下一步反应做准备）。得到Fmoc-Tyr(tBu)-Linker-MBHA Resin。氨基酸：DIPEA：HBTU：树脂=3：6：2.85：1（摩尔比）。结构图如下：
4、依次循环步骤二、步骤三，依次得到
H2N-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Pro-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Pro-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Lys(Boc)-Pro-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
H2N-Lys(Boc)-Pro-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
Fmoc-Ala-Lys(Boc)-Pro-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin
以上中间结构，均可在专肽生物多肽计算器-多肽结构计算器中，一键画出。
最后再经过步骤二得到 H2N-Ala-Lys(Boc)-Pro-Glu(OtBu)-Ala-Pro-Gly-Glu(OtBu)-Asp(OtBu)-Ala-Ser(tBu)-Pro-Glu(OtBu)-Glu(OtBu)-Leu-Ser(tBu)-Arg(Pbf)-Tyr(tBu)-Tyr(tBu)-Ala-Ser(tBu)-Leu-Arg(Pbf)-His(Trt)-Tyr(tBu)-Leu-Asn(Trt)-Leu-Val-Thr(tBu)-Arg(Pbf)-Gln(Trt)-Arg(Pbf)-Tyr(tBu)-Linker-MBHA Resin，结构如下：
5、切割：6倍树脂体积的切割液（或每1g树脂加8ml左右的切割液），摇床摇晃 2小时，过滤掉树脂，用冰无水乙醚沉淀滤液，并用冰无水乙醚洗涤沉淀物3次，最后将沉淀物放真空干燥釜中，常温干燥24小试，得到粗品H2N-Ala-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2。结构图见产品结构图。
切割液选择：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前两种适合没有容易氧化的氨基酸，例如Trp、Cys、Met。第三种适合几乎所有的序列。）
6、纯化冻干：使用液相色谱纯化，收集目标峰液体，进行冻干，获得蓬松的粉末状固体多肽。不过这时要取小样复测下纯度 是否目标纯度。
7、最后总结：
杭州专肽生物技术有限公司（ALLPEPTIDE https://www.allpeptide.com）主营定制多肽合成业务，提供各类长肽，短肽，环肽，提供各类修饰肽，如：荧光标记修饰（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基团修饰肽（叠氮、炔基、DBCO、DOTA、NOTA等），同位素标记肽（N15、C13），订书肽（Stapled Peptide）,脂肪酸修饰肽（Pal、Myr、Ste），磷酸化修饰肽（P-Ser、P-Thr、P-Tyr），环肽（酰胺键环肽、一对或者多对二硫键环），生物素标记肽，PEG修饰肽，甲基化修饰肽等。
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